Background:
: Lupinus angustifolius L also known as narrow-leafed lupin (NLL) is becoming an important grain legume crop that is valuable for sustainable farming and is becoming recognised as a human health food. Recent interest is being directed at NLL to improve grain production, disease and pest management and health benefits. However, studies have been hindered by a lack of extensive genomic resources for the species.
Results:
: A NLL BAC library was constructed consisting of 111,360 clones with an average insert size of 99.7 Kbp from cv Tanjil. The library has approximately 12 X genome coverage. Both ends of 9600 randomly selected BAC clones were sequenced to generate 13985 BAC end-sequences (BESs), covering approximately 1% of the NLL genome. These BESs permitted a preliminary characterisation of the NLL genome such as organisation and composition, with the BESs having approximately 39% G:C content, 16.6% repetitive DNA and 5.4% putative gene-encoding regions. From the BESs we identified 9966 simple sequence repeat (SSR) motifs and some of these are shown to be potential markers.
Conclusions:
: The NLL BAC library and BAC-end sequences are powerful resources for genetic and genomic research on lupin. These resources will provide a robust platform for future high-resolution mapping, map-based cloning, comparative genomics and assembly of whole-genome sequencing data for this species.
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